Immobilised Enzymes Laboratory
Immobilised Enzymes Laboratory Introduction: In this experiment alkaline phosphatase will be immobilised in a variety of methods to show the variation of activity of each method and also to show the continuing activity of each method after successive tests. ... The three methods that will be used are going to be: Surface Immobilisation Polyacrylamide Gel Alginate Gel These methods will be compared to the activity of the enzyme when not immobilised, i. ... Surface Immobilised Enzyme Assay The surfaces used were cover slips and onto one went 100無 of enzyme and onto the other went a mixture of 100無 of enzyme mixed with 100無 of glutaraldehyde. ... 080 It can be seen from the results that the enzyme on its own immobilised onto the cover slip had an activity similar to that of the free enzyme. ... The enzymes that were covalently bound to each other, by the use of glutaraldehyde, had an activity lower than that of the surface immobilised enzyme.