effect of temperature on catalase
"Effect on temperature on catalase" Extended Title: "have to draw graphs yourself" Designing the Experiment Skill Area P Aim To find the rate of reaction of Catalase in potato with Hydrogen peroxide when changing the temperature, i. ... Prediction I think that when the temperature is increased the rate of reaction will increase i. ... I think that beyond a certain temperature the reaction will not increase any more and then decrease rapidly because the enzymes will start to be denatured. ... I also believe that up to 40°C if the temperature is increased by 10°C the rate will be doubled. ... But this takes a long time so catalase in the potato is used to speed up the reaction. The catalase is an enzyme, enzymes are catalysts which control the speeds of chemical reactions in cells, which lower the activation energy needed for particles to react, so that they will react more easily and are sensitive to changes in temperature. The increase in temperature gives more activation energy so that the particles of hydrogen peroxide and the Catalase enzymes collide with each other more often. (An increase in pH also does the same as temperature.) The activation energy is a barrier which the reactants (catalase and hydrogen peroxide) must climb before they can convert into the products. ... The reason why the rate will double when there is an increase in 10°C if the temperature is up to 40°C is because of the Arranius equation, which is: rate = Ae -EACT RT Special receptors on the surface of the catalase are in a special shape which enables the hydrogen peroxide molecule to fit into it and cause it to become broken, speeding up the reaction. The catalase (or other enzymes) cause a process were big molecules are broken down into smaller ones, this is called Catabolic. If the temperature is too high the enzymes will be denatured because the special receptors will become deformed and the particles of the hydrogen peroxide wont fit into it anymore therefore the molecules will not be broken. Key Factors The factors which are possible to change in this experiment are: • Concentration of hydrogen peroxide • Volume of hydrogen peroxide • The temperature • Surface area of the potato • The pH • Type of potato • Method of oxygen collection • Mass of the potato Fair Test The factors that I will need to control are: • The volume of the hydrogen peroxide • The concentration of the hydrogen peroxide • The method of oxygen collection • The pH • The surface area of the potato • The mass of the potato • The type of potato Plan Apparatus: • Potatoes • 25ml Measuring cylinder • Boiling tube (x2) • Rubber tube • Beaker • Bung • Hydrogen peroxide of 20volIce (approx. ... The boiling tubes should be placed in the temperature of the test that your about to carry out i.